Coding

Part:BBa_K2152001:Design

Designed by: Dekang Li   Group: iGEM16_ShanghaiTechChina_B   (2016-10-13)


human Epidermal Growth Factor（hEGF） with FLAG-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a phoA signal peptide at the N-terminus and a FLAG tag at the C-terminus of hEGF protein. PhoA is a signal peptide that can guide protein to secrete to periplasmic space in E.coli. We can detect the FLAG tag on the protein by western blotting.


Source

hEGF sequence comes from human genome and is synthesized by GenScript.

References